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Amerigo Scientific

MAL-dPEG®₄-TFP ester | Amerigo Scientific

MAL-dPEG®4-TFP ester, product number 10551, is a crosslinking reagent that joins free amines to free thiols (sulfhydryl groups). The sulfhydryl groups react with a maleimido group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the 2,3,5,6-tetrafluorophenyl (TFP) ester of the terminal propionic acid group. The maleimido and TFP functional groups on the crosslinking compound sit at either end of a short, discrete chain of polyethylene glycol (hence, dPEG®).

Crosslinking Reactions

One of the most popular, most useful crosslinking reactions in bioconjugate chemistry[1],[2] is the conjugation of free amines to free thiols. These reactions require heterobifunctional reagents that bridge the two groups. Typical crosslinkers are hydrophobic. Our dPEG® crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths.

With conventional hydrophobic crosslinking reagents, aggregation and precipitation of the conjugates occur frequently. These problems do not happen with our water-soluble, non-immunogenic dPEG® crosslinkers.

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ABOUT THE COMPANY

Amerigo Scientific is a distribution company focused on providing critical products and services to the biomedical and life science research communities. Although a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in...
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